Abstract
Mesenchymal stem cells (MSCs) play a key role in wound healing, and the advantages
of pretreated MSCs in wound healing have previously been reported. In the present
study, we investigated the impact of LPS pretreated human adipose-derived MSCs on
skin wound healing in diabetic rats. We found that some improvements occurred through
improving angiogenesis. Then, we scrutinized the impact of lipopolysaccharide (LPS)
treatment on human adipose-derived MSCs in a high-glucose (HG) medium, as an in vitro diabetic model. In vivo findings revealed significant improvements in epithelialization and angiogenesis
of diabetic wounds which received LPS pre-MSCs. Particularly, LPS pre-MSCs-treated
diabetic wounds reached considerably higher percentages of wound closure. Also, the
granulation tissue of these wounds had higher pronounced epithelialization and more
vascularization compared with PBS-treated and MSCs-treated diabetic ones by CD31,
VEGF, CD90, collagen 1, and collagen 3 immunostaining. Western-blots analyses indicated
that LPS pre-MSCs led to the upregulation of vascular endothelial growth factor (VEGF)
and DNMT1. In addition, significantly higher cell viability (proliferation/colonie),
and elevated VEGF and DNMT1 protein expression were observed when MSCs were treated
with LPS (10 ng/ml, 6 h) in HG culture media. Based on these findings, it is suggested
that LPS pre-MSCs could promote wound repair and skin regeneration, in some major
processes, via the improvement of cellular behaviors of MSCs in the diabetic microenvironment.
The beneficial advantages of LPS treated with mesenchymal stem cells on wound healing
may lead to establishing a novel approach as an alternative therapeutic procedure
to cure chronic wounds in diabetic conditions.
Keywords
Abbreviations:
MSCs (Mesenchymal stem cells), LPS (Lipopolysaccharide), DNMT1 (DNA methyl transferase 1), HG (high-glucose), VEGF (Vascular endothelial growth factor), ECM (Extracellular matrix), HGF (Hepatocyte growth factor), bFGF (basic fibroblast growth factor), TGF-β (Transforminggrowthfactor-β), IGF-1 (Insulin-like growth factor 1)To read this article in full you will need to make a payment
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Article info
Publication history
Published online: September 24, 2022
Accepted:
September 23,
2022
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© 2022 Published by Elsevier Ltd.
