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Growth Differentiation Factor 7 promotes multiple-lineage differentiation in tenogenic cultures of mesenchymal stem cells

  • Maritha Kumlin
    Correspondence
    Corresponding author.
    Affiliations
    Trauma and Reparative Medicine, Karolinska University Hospital, Stockholm, Sweden

    The Division of Orthopedics and Biotechnology, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, Stockholm, Sweden
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  • Karolina Lindberg
    Affiliations
    The Division of Orthopedics and Biotechnology, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, Stockholm, Sweden
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  • Lars-Arne Haldosen
    Affiliations
    Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden
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  • Li Felländer-Tsai
    Affiliations
    Trauma and Reparative Medicine, Karolinska University Hospital, Stockholm, Sweden

    The Division of Orthopedics and Biotechnology, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, Stockholm, Sweden
    Search for articles by this author
  • Yan Li
    Affiliations
    Trauma and Reparative Medicine, Karolinska University Hospital, Stockholm, Sweden

    The Division of Orthopedics and Biotechnology, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, Stockholm, Sweden
    Search for articles by this author
Published:September 16, 2022DOI:https://doi.org/10.1016/j.injury.2022.09.017

      Highlights

      • This study describes an in vitro model with murine stem cells (C3H10) for the study of tenogenic differentiation.
      • Growth differentiation factor 7 (GDF-7) stimulates tenogenic differentiation in this model.
      • GDF-7 also stimulates adipogenic and osteogenic differentiation.

      Abstract

      The repair of the tendon-bone interface, which is composed of tendon, fibrocartilage, and bony attachment, remains a clinical challenge. The application of mesenchymal stem cells (MSCs), collagen-rich extracellular matrix (ECMs), as well as growth factors, has the potential to regenerate this special multiple-tissue structure through the so-called biological augmentation. We present here an in vitro tendon regeneration model with C3H10T1/2 cells cultured on Collagen I matrix and evaluated the lineage determination effects of Growth Differentiation Factor 7 (GDF-7). We found that besides tenogenic effect, GDF-7 also stimulates the expression of osteoblastic as well as adipocytic genes. Our results indicate that GDF-7 might be a promising growth factor for regeneration of the tendon-bone interface due to its multiple-lineage stimulating effects. However, the side effect on adipogenic differentiation should be of concern, as it is a known risk factor for repair failures.

      Keywords

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