Highlights
- •This study describes an in vitro model with murine stem cells (C3H10) for the study of tenogenic differentiation.
- •Growth differentiation factor 7 (GDF-7) stimulates tenogenic differentiation in this model.
- •GDF-7 also stimulates adipogenic and osteogenic differentiation.
Abstract
The repair of the tendon-bone interface, which is composed of tendon, fibrocartilage,
and bony attachment, remains a clinical challenge. The application of mesenchymal
stem cells (MSCs), collagen-rich extracellular matrix (ECMs), as well as growth factors,
has the potential to regenerate this special multiple-tissue structure through the
so-called biological augmentation. We present here an in vitro tendon regeneration
model with C3H10T1/2 cells cultured on Collagen I matrix and evaluated the lineage
determination effects of Growth Differentiation Factor 7 (GDF-7). We found that besides
tenogenic effect, GDF-7 also stimulates the expression of osteoblastic as well as
adipocytic genes. Our results indicate that GDF-7 might be a promising growth factor
for regeneration of the tendon-bone interface due to its multiple-lineage stimulating
effects. However, the side effect on adipogenic differentiation should be of concern,
as it is a known risk factor for repair failures.
Keywords
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Article info
Publication history
Published online: September 16, 2022
Accepted:
September 11,
2022
Identification
Copyright
© 2022 Published by Elsevier Ltd.
