Highlights
- •Lyophilized equine platelets derived growth factor (LGF) could repair chondoral defect.
- •LGF lowered the pro-inflammatory cytokines; TNF-α and IL-1β.
- •LGF increased the anabolic and angiogenic growth factors; PDGF and TGF-β1.
- •LGF elevated the expression of chondrogenic-related marker genes; Col I and Col II.
- •LGF decreased the immune-expression of NF-κB p65 and MMP2 proteins.
- •LGF improved the histopathological alterations in osteoarthritis.
Abstract
Lyophilized equine platelet derived growth factors (LGF) is a novel advanced platelet
rich protein growth factor. It has been successfully applied in various fields of
regenerative medicine to treat a variety of inflammatory and degenerative musculoskeletal
conditions. Our study aimed to evaluate the efficacy of intraarticularly injected
LGF for the remedy of articular cartilage injury, commonly characterized by progressive
pain and loss of joint function in osteoarthritic rabbits. Full-thickness cylindrical
cartilage defects were generated in both femoral condylar articular surfaces in twenty
rabbits. The left joint of all animals was injected with the adjuvant as a self-control
negative, while the right joint was injected by LGF. Four- and eight-weeks post-surgery,
the femoral condyles were harvested, and assessed grossly, microscopically and immunohistochemically.
Cytokines (TNF-α, IL-1β, PDGF and TGF-β1) contents of the chondral defects were quantified
by ELISA as well as the gene expression of Col I and Col II via RT-qPCR. The LGF treated
defects showed significant higher ICRS (International cartilage repair society) healing
scores of cartilaginous regeneration with a significant higher histological healing
score on using O'Driscoll histological scoring system. Additionally, LGF significantly
lowered the levels of the pro-inflammatory cytokines TNF-α and IL-1β. It also significantly
increased the anabolic and angiogenic growth factors (PDGF and TGF-β1), and significantly
elevated the expression of chondrogenic-related marker genes; Col I and Col II. The
current study reveals that LGF improves chondral healing and thus it can be a superior
nominee as an adjunctive therapy to positively influence regeneration of chondral
defects in osteoarthritic patients.
Keywords
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Article info
Publication history
Published online: February 05, 2022
Accepted:
February 3,
2022
Identification
Copyright
© 2022 Elsevier Ltd. All rights reserved.
