Abstract
For tissue engineering of critical size bone grafts, nanocomposites are getting more
and more attractive due to their controllable physical and biological properties.
We report in vitro and in vivo behaviour of an electrospun nanocomposite based on poly-lactic-co-glycolic acid and
amorphous calcium phosphate nanoparticles (PLGA/a-CaP) seeded with human adipose-derived
stem cells (ASC) compared to PLGA. Major findings were that cell attachment, three-dimensional
ingrowth and proliferation were very good on both materials. Cell morphology changed
from a spindle-shaped fibroblast-like form to a more roundish type when ASC were seeded
on PLGA, while they retained their morphology on PLGA/a-CaP. Moreover, we found ASC
differentiation to a phenotype committed towards osteogenesis when a-CaP nanoparticles
were suspended in normal culture medium without any osteogenic supplements, which
renders a-CaP nanoparticles an interesting osteoinductive component for the synthesis
of other nanocomposites than PLGA/a-CaP. Finally, electrospun PLGA/a-CaP scaffold
architecture is suitable for a rapid and homogenous vascularisation confirmed by a
complete penetration by avian vessels from the chick chorioallantoic membrane (CAM)
within one week.
Keywords
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Article info
Publication history
Accepted:
June 1,
2012
Footnotes
☆The work was performed at the University Hospital Zurich, ZKF, Sternwartstrasse 14, 8091 Zurich, Switzerland.
Identification
Copyright
© 2012 Elsevier Ltd. Published by Elsevier Inc. All rights reserved.
